In my biology lab, we used this technique to determine the charge of the protein by creating a gelatinous substance, punching small holes in it, and then carefully inserting various proteins in each one. From there, a positive electrode (cathode) was connected to one side, and a negative electrode to the other (anode). The distance that each protein traveled along the surface (covered in a liquid) determined it's charge. Naturally, the positively charged proteins moved toward the negative end, and the negatively charged proteins moved towards the positive end. That's electrophoresis in a nutshell - from my limited experience. For more details, try wikipedia.
2007-02-13 14:49:58
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answer #1
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answered by Clean Independent Energy 3
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Electrophoresis is used to separate molecules by applying an electric current to a gel containing the molecules. It's commonly used to analyze DNA and proteins.
It's like the electrical equivalent of chromatography.
2007-02-13 14:47:26
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answer #2
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answered by ecolink 7
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this is a technique used to separate the molecules that may be protein or DNA on the basis of size, charge and other properties. In this case electric current is applied and small molecules will move faster and larger will follow them.
for more information about electrophoresis see following website.
http://www.bergen.org/AAST/projects/Gel
2007-02-13 14:54:41
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answer #3
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answered by jatinder 1
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ooo! i think i can tell u! lol
if u have a sample of dna and u want to elongate a specific sequence you first do something called PCR. Then you place your dna samples in a gel and run the gel at a specific voltage...the bands will seperate based on size and charge...depeding on what marker you use you can determine which bands you have...
this process is electrophoresis
2007-02-13 14:48:55
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answer #4
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answered by hgiggy 2
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It is used to separate DNA fragments based on size and charge. It can be used to compare the size of the fragements with known markers. It is also called DNA Fingerprinting.
2007-02-13 19:50:46
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answer #5
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answered by Linnea L 3
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http://dictionary.reference.com/search?q=Electrophoresis
2007-02-13 14:49:51
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answer #6
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answered by Anonymous
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