Why is ADP used as a ligand in affinity column chromatography for cytochrome P-450BM3?

Question

I understand that ADP is a ligand for this enzyme, but how/why does the enzyme use ADP?

Answer 1

According to Wiki: Cytochrome P450BM3 catalyzes the NADPH-dependent hydroxylation of several long-chain fatty acids at the ω–1 through ω–3 positions.

So (if I may dust off my knowledge of biochem) I suggest you look at the structure of NADP (it is ADP with a nicotinamide) so the actual substrate is NADP but ADP works to bind it to an affinity column.

This is a good site to revie wthe mechanism of P450BM3
http://www.uky.edu/Pharmacy/ps/porter/CPR_enzymology.htm

but the key in your question is to know that ADP is a main structural component of nADPh which is why this works as a purification tool.

Answer 2

And you can elute p450 from teh column with free ADP