why we need to centrifuge the competent cell at temperature 4C?

2007-02-12 20:05:51 · 1 answers · asked by teck kim 2 in Science & Mathematics ➔ Biology

1 answers

The cells need to be kept cool until they are ready to accept the plasmid you are transforming them with. If you allow them to warm up, they will start to grow and fewer will be competent and that will lower your transformation efficiency.

2007-02-13 01:58:04 · answer #1 · answered by btpage0630 5 · 0⤊ 0⤋

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