how can one apply the methods of paper chromatography in the identification of unknown amino acid?

Answer 1

Paper chromatography may not be the best method. There are electrophoresis and gel filtration chromatography, for example.

Electrophoresis uses a electrical potential across a gel to separate by charge density. Small amino acids move quicker than large ones. Amino acids with larger charges move quicker.

In gel filtration chromatography larger particles move quicker than smaller particles which be caught in the pores.