what is vacuum infiltration?

Answer 1

Do you mean Vacuum infiltration of Arabidopsis? If so then, Vacuum infiltration of Arabidopsis was developed as the first method to bypass tissue culture entirely. Here, the developing floral meristems/flowers are placed in an Agrobacterium solution under vacuum, and germ cells are transformed. The plant is grown out and allowed to set seed. Potentially, each new seed that is collected represents an independent transformant. This method has been used to produce large numbers of T-DNA insertion mutagenized Arabidopsisplants . More recently, the vacuum step has been found unnecessary and that simply dipping flowers in Agrobacterium solution is sufficient to transform cells. While there have been attempts to use this methodology on other plants species, there are no published successes to date.

Answer 2

Infiltration means going inside and vacuum infiltration means getting something to fill up the spaces inside another object by removing the air. Powder Metallurgy products like bearings have a large volume of pores and these pores can be infiltrated with lubricating oil by applying to vacuum first and then admitting oil into the system. Such bearings will be self-lubricating for life. It is one example.