I am curious if I can extract DNA from blood rather then tissue for rodentia? I am going to start researching an endagered species and am having to jump through hoops to get clearance to simply extract blood. What is the difference in the amount of DNA from blood compared to organ tissue?
2007-02-09 08:44:31 · 3 answers · asked by motobecane 2 in Science & Mathematics ➔ Biology
Yes you can extract DNA from blood rather than tissue, but the difference is that cells in the blood are mostly RBC and they are annucleated, the DNA would have comes from white cells and possibly endothelial cells, that means you will need more blood to obtain the same amount of DNA from tissue in terms of volume. so i would recommend you extract the blood and centrifugage it and take the white cell component only, than lyse the cells apply proteinase, RNAase and other routine procedures.
2007-02-09 08:56:39 · answer #1 · answered by lippy19850528 3 · 0⤊ 0⤋
Extracting DNA from blood is done all the time in our laboratory for PCR genotype verification in our transgenic mouse colony.
Depends on your institution, but I suspect simple blood extraction will require IACUC approval. Welcome to academia and all the associated hoop-jumping.
2007-02-09 08:52:12 · answer #2 · answered by leprechaun 2 · 0⤊ 0⤋
When DNA extractions are performed, you can expect three basic results.
1. No DNA
2. DNA appears fluffy which means it has sheared in the extraction process
3. DNA appears as thin threads.
Although DNA that strands is the most impressive, DNA that has sheared still shows that DNA is present.
2007-02-09 08:52:30 · answer #3 · answered by Vasif Baig 4 · 0⤊ 2⤋