LIGHT MICROSCOPE:
Light microscopes shine light through a specimen and into an eyepiece for you to look at.
An electron microscope sprays electrons as a speciment in a vacuum. Data is collected on how the electrons reflect off of the specimen.
RESOLUTION:
The magnification of small things is a necessary facet of biological research, but the fine detail in cells and in subcellular components requires that any imaging system be capable of providing spatial information across small distances. Resolution is defined as the ability to distinguish two very small and closely-spaced objects as separate entities. Resolution is best when the distance separating the two tiny objects is small. Resolution is determined by certain physical parameters that include the wavelength of light, and the light-gathering power of the objective and condenser lenses. A simple mathematical equation defines the smallest distance (d min ) separating the two very small objects:
dmin = 1.
2007-02-07
19:37:44
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2 answers
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asked by
churchill p
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Science & Mathematics
➔ Biology