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LIGHT MICROSCOPE:
Light microscopes shine light through a specimen and into an eyepiece for you to look at.
An electron microscope sprays electrons as a speciment in a vacuum. Data is collected on how the electrons reflect off of the specimen.
RESOLUTION:
The magnification of small things is a necessary facet of biological research, but the fine detail in cells and in subcellular components requires that any imaging system be capable of providing spatial information across small distances. Resolution is defined as the ability to distinguish two very small and closely-spaced objects as separate entities. Resolution is best when the distance separating the two tiny objects is small. Resolution is determined by certain physical parameters that include the wavelength of light, and the light-gathering power of the objective and condenser lenses. A simple mathematical equation defines the smallest distance (d min ) separating the two very small objects:
dmin = 1.

2007-02-07 19:37:44 · 2 answers · asked by churchill p 1 in Science & Mathematics Biology

2 answers

Pretty much correct.
Probably should include more notes on the two types of electron microscopes (Transmission and scanning).

2007-02-10 07:54:04 · answer #1 · answered by kz 4 · 0 0

Basically this is what they mainly ask in exams- optic microscope (ordinary microscope) uses long wavelengths, and a lens with a magnifier in, so you can see objects but not as well as an electron microscope as an electron microscope has a smaller wavelength, which creates a higher resolution(the ability to distinguish between two close objects) there's also 2 types of electron microscope, transmission and scanning. we did this in September so its good revision for me :) a transmission microscope uses a small cross section of the sample, so it has to be dead, that's a downside to it. It sends beams of electrons to the sample and it produces the image on a photographic plate.You are able to see the ultra cellular organelles, in a 2D image on a monitor, its very Hi tech so schools an colleges don't use it! Scanning microscope can produce a better quality 3D picture. hope this is some help, some revision for me :)

2016-03-28 21:49:43 · answer #2 · answered by Anonymous · 0 0

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