Use of saline instead of water?

Question

I had a lab in Bio the other day and we were unable to complete the lab, I can't find this answer anywhere. Explain why a 0.87% sakube was used with human epithelial cells instead of tap water. Whats the possible answer to that?

* SALINE...sorry!

Answer 1

Saline is isotonic with respect to the cell.

A cell can be thought of a bag of salty solutions. The membrane is semi-permeable, meaning it allows water to pass through easily, but not everything. If the cells were put into water, osmotic pressure would cause water to enter the cell (in an attempt to equalize the concentration of the salts within and without the cell). The cell would ultimately burst.

Using saline allows the osmotic pressure across the membrane to be close to zero. The cell remains intact.

For a demonstration, put a stalk of celery in distilled water and one stalk in salty water (saline). The one in distilled water will appear very rigid as water has gone into the cells. The one in salty water will be flaccid as water has escaped.

Answer 2

tap water is full of contaminates, that could give false results, saline , under lab conditions is likely to be sterilised salt water, so few possible contaminates to interfere with the result.

Answer 3

its probably phoshored buffered saline. its gentle to the cells and acts as a detergent. it used as a buffer for a range of molecualr biology applications.

Answer 4

i here by say that you are weak in biology its not your fault its the lab assistants he might be playing pranks on you
your labassistant wants to show off that he s a test tube baby
thats all

Answer 5

7.2 PH NaCl