how to count cells after DAPI staining?

Answer 1

DAPI stains cells by binding to the DNA in the nucleus. After DAPI staining each nucleus should be clearly visible, and assuming you're working with a normal eukaryote your nuclei count should equal your cell count. DAPI flouresces at 365 nm when excited by light at 350nm, so you'll need a flourescent scope with a narrow band filter.

Answer 2

You need to get a blacklight yourself (you can usually rent them from a pet store, or buy a cheap one at walmart) and go over the rug first to find any stains. Then you need to use a pet stain/odor remover that is enzyme based. That is the only kind of cleaner that will reliably remove that kind of stain. Nature's Miracle is one brand, but if you go to the pet store and ask for an enzyme-based cleaner they should be able to help you. Be sure to check the label to make sure that there are no added perfumes or anything like that. Thoroughly soak any areas that show up with your blacklight. Leave it sit for as long as it says on the label, or half an hour if it doesn't say. Then soak up all the liquid with a towel or paper towels. Dry the area thoroughly and then check it with the blacklight. You may have to repeat more than once to get an old stain out. Always be sure to soak the area thoroughly, no matter what it says on the bottle. If the liquid does not reach the underpadding of the carpet, the stain will remain and perhaps encourage the puppy to use the same spot again. AVOID as much as you can using any other kind of cleaners on the area beforehand - vinegar, diluted bleach, and non-enzyme based pet stain cleaners may actually set the stain. If all else fails, you can always spill wine or juice on the stained areas to disguise their true nature :P Be sure to check your local by-laws to make sure that they really do have the right to evict you if any stains show up. Your local landlord-tenant agency may be able to help you.