what are the advantage/disadvantage between Michaelis-Menten,Lineweaver-Burk Plot to get the Vmax and Km.?

Question

i have read from internet, and know that Lineweaver-Burk Plot will suggest some inaccuracy in the determination of the Vmax and Km, but why we still use it, i think that it must have some advantage to use Lineweaver-Burk Plot. so need some one to tell me? sorry my english is not good enough.

Answer 1

The Lineweaver-Burk plot was widely used to determine important terms in enzyme kinetics, such as Km and Vmax before the wide availability of powerful computers and non-linear regression software, as the y-intercept of such a graph is equivalent to the inverse of Vmax; the x-intercept of the graph represents -1/Km. It also gives a quick, visual impression of the different forms of enzyme inhibition.

The double reciprocal plot distorts the error structure of the data, and it is therefore unreliable for the determination of enzyme kinetic parameters. Although it is still used for representation of kinetic data[2], non-linear regression or alternative linear forms of the Michaelis-Menten equation such as the Eadie-Hofstee plot are generally used for the calculation of parameters[3].