37 Celsius is body temperature of humans. Thus, E. coli, the favorite lab rat of molecular biologists, which lives inside humans, would have an optimum temperature of 37 Celsius.
2007-02-10 08:39:24
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answer #1
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answered by kz 4
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Well the significance is tied in to the whole incubation process.
One needs to keep cultures at an optimal temperature and humidity. If it is really important to your project, you probably need to get a hold of something to regulate the carbon dioxide(CO2) and oxygen content of the atmosphere; thus you could buy a CO2 incubator.
Cell incubators are essential for a lot of experimental work in cell biology and molecular biology and are used to culture both bacterial as well as eukaryotic cells.
For mammalian cells, the chamber temperature is typically set to 37 °C, the relative humidity is >95% and a slightly acidic pH is achieved by maintaining a CO2 level of 5%. Thus keeping optimal temperature and humidity for cellular growth.
Note: this is NOT done just to avoid other things growing growing in your culture. Hopefully you've sterilized your tools PRIOR to culturing the organism; thus avoiding contamination.
2007-02-06 16:56:33
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answer #2
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answered by Hmmm 2
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TSA plates are incubated at 37C because, that is the ideal temperature for your cultures to grow. 37C has a significance in that it is the same temperature we are internally. So if you are culturing E. coli for example (which causes disease in humans) you simulate that same environment in the incubator.
2007-02-06 17:54:20
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answer #3
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answered by cristy l 2
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That is the optimum growing temperature for bacteria - namely E. coli which is one of the most widely used scientific bacteria in the world.
2007-02-06 16:48:13
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answer #4
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answered by gman1602 3
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That is human body temperature, if you're looking for human pathogens, that's where you start.
2007-02-06 18:03:25
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answer #5
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answered by lizettadf 4
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preventing growth without killing the specimen.
2007-02-06 16:47:23
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answer #6
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answered by User Name 5
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