In testing the specificity of enzyme action,what are the roles of NaCl and the phosphate buffer?

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In testing the specificity of enzyme action,what are the roles of NaCl and the phosphate buffer?

2007-02-05 17:55:58 · 2 answers · asked by amazing_sweet_angel 1 in Science & Mathematics ➔ Chemistry

2 answers

Enzymes are very pH sensitive. NaCl and phosphate buffer are used to control the pH of the media to ensure the right pH for the desired action.

2007-02-05 19:03:06 · answer #1 · answered by azorysky 1 · 0⤊ 0⤋

Being proteins, enzymes are also sensitive to dissolved salts as well as pH. The buffer solutions most commonly used are designed to mimic the pH and salt concentration found in blood.

2007-02-08 13:15:34 · answer #2 · answered by michael h 1 · 1⤊ 0⤋