2007-02-04 17:19:12 · 4 answers · asked by aida aadzrian 1 in Science & Mathematics ➔ Biology
The unwanted molecule is not the DNA. You wouldn't use restriction enzymes in a DNA extraction. For that matter, EDTA does not break up cells.
You need some form of soap or detergent to break up membranes, and you need either protease or phenol/chloroform or anionic columns to separate the DNA from unwanted proteins and cellular debris.
2007-02-05 01:31:48 · answer #1 · answered by floundering penguins 5 · 0⤊ 0⤋
EDTA for cell breakage
Phenol chloroform to seperate the dna-proteins
precipitate and store in alcohol
2007-02-05 02:23:40 · answer #2 · answered by kala b 2 · 0⤊ 1⤋
Agent: restriction enzyme.
Process: electropherosis.
2007-02-05 06:49:11 · answer #3 · answered by Valeri 2 · 0⤊ 1⤋
Rescrition enzymes cuts the parts, you want, out
It is all part of recombinant DNA,
2007-02-05 02:18:09 · answer #4 · answered by -Eugenious- 3 · 0⤊ 2⤋