A gene technolegy that magnifies the amount of DNA available?

Question

Biology

Answer 1

the common and most effective one is PCR, or polymerase chain reaction. With this, you can amplify a DNA sample millions of times within a few hours.

Basically what you do is you stick the DNA sample in a solution containing free nucleotides and DNA polymerases, along with synthetic primers, and then u let it run in heating and cooling cycles to let the strands separate, and then for the primers and DNA polymerases to do their job

what can also be done is cloning the piece of DNA and inserting it as a recombinant plasmid in a bacterial cell, and then letting the bacteria replicated

Answer 2

PCR - polymerase chain reaction
can amplify sections of DNA exponentially. normally not much more than 5-6kb
uses part of the DNA sequence as a template, uses small approx 20-mer sequences as primers and a heat tolerant DNA polymerase (taq) to amplify the sequence. this occurs with cycles which allow the primers to bind, allow the taq to amplfy then allow the primers and new sequence to separate. the sequence produced acts as a template in the next round of amplification.

Answer 3

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