Using a spectrophotometer, is a 1:5 dilution absorbed more than a 1:10 dilution?

Answer 1

1:5 solution absorbs more than then the 1:10 solution.

Beer's law: A = a b c.
As concentration goes up, absorbance goes up proportionally.

Answer 2

a 1/5 dilution is twice as concentrated as 1/10. so it absorbs more the light

Answer 3

Concentration will always affect the absorption. Higher concentration - more intense absorption. There is a limit to this, where the absorption/emission will be maxed out at high concentration. In other words, your peaks will be very broad, very intense, and tell you absolutely nothing.

Generally, lower concentrations are better to use in spectroscopic measurements for this reason.