what is the function of the solutions(GTE, KCH3cooh..) listed below in the plasmid extration?

Question

: what is GTE, TE buffer and ethanol?and the fuction of it in plasmid extration? function of potassium acetate in plasmid extration?

what is the function for glucose, tris and EDTA buffer solution (GTE), ethanol and TE buffer, potassium acetate, 0.2M NaOH/ 1% SDS isoprapanol in plasmid extration. instantly i just know some of its function, i more precise to know how them help in plasmid extration

Answer 1

GTE is a glucose, tris and EDTA buffer solution.
TE is a tris/EDTA buffer solution
ethanol is an alcohol, C2H5OH.
The ethanol can be used to precipitate the DNA present in your bacteria/yeast. The TE buffer is used to create a nice pH level (Tris buffer) , and to catch all Mg and/or Mn ions in solution, to prevent DNAse from working properly. The DNAse which just "eats" DNA needs these ions for working.