why tris buffer is only used in PCR?

Answer 1

TRIS buffer is used in lots of applications, because it is a good buffer (maintains pH well).

Answer 2

Tris is used to maintain the PCR reaction at the proper pH. Different polymerases will have different pH levels of tris in the buffer due to properties of different polymerases and buffers. Some PCR optimization kits offer buffers with varying buffer additives, including various concentrations and pH of tris. Tris. or Tris-HCl, is also used for storage of the buffer.

Answer 3

It may be used in older protocols but not the high temperature PCR used for research with an effective pH range between 6.5 and 9.7 and most effective at 20 degrees celsius, Are you sure you are not talking about agarose electrophoresis?

First, you must understand that the PCR you perform in HS science or even College science labs is not the same protocol the FBI or say Roche Molecular sciences uses You are using a old protocol and it is not performed in such an assembly line fashion and you are not using the higher temperatures unless you are using Taq Polymerase, because it is patented and unless you have a grant and are a noted scientist your facility will not pay the royalties for such research

There are a number of buffers used for agarose electrophoresis. The most common being: TRIS acetate EDTA (TAE), Tris/Borate/EDTA (TBE)