How do i find the isoelectric point from a titration curve? How can i tell what the pKa values for COOH and NH3+ using the titration curve?
2007-01-28 09:45:12 · 2 answers · asked by ginnyp82 2 in Science & Mathematics ➔ Chemistry
If you're dealing with amino acids (I'm guessing that's the case), and IF there are only two ionizable groups in the molecule, then the isoelectric point is just the average of the two pKa's. The lower pKa is going to be for the carboxyl group (it is an acidic group) and the higher pKa will corresond to the amino group (it'll always be in the basic region).
You'll probably need to be able to describe the net charge of an amino acid at different points in the titration curve. Most biochem texts will discuss this pretty well. That'll become important when you deal with the titration curve and isoelectric points of amino acids with a 3rd ionizable group...
2007-01-28 09:54:39 · answer #1 · answered by hcbiochem 7 · 0⤊ 0⤋
sds
2007-01-28 17:47:51 · answer #2 · answered by princetongirl81824 2 · 0⤊ 0⤋