You are given a broth culture that is growing an unknown organism. What is the first test you do and why?
2007-01-28 07:03:37 · 3 answers · asked by Steenskees 2 in Science & Mathematics ➔ Biology
gram stain the broth, and see if it's gram neg or pos, bacilli or cocci, or a mixture of organisms. then plate the broth to blood agar and chocolate agar, and macconkey's if you have a gram neg rod. if the specimen is from a wound or a sterile body fluid, be sure to incubate a blood agar plate anaerobically to rule out anaerobes. after that, you'll be able to narrow it down and test for the specific organisms that have the characteristics you find.
2007-01-28 09:59:13 · answer #1 · answered by joshstew85 2 · 0⤊ 0⤋
Attempt to identify the organism. Look for important classes of characteristics such as:
size
type of margin
colony elevation
colony texture
light transmission
colony pigmentation
These characteristics will help you identify the type of organism you are dealing with.
2007-01-28 07:12:26 · answer #2 · answered by michelle 5 · 0⤊ 0⤋
Make a slide and stain it and view it. Is it gram neg or pos. What is the shape of the organism?
2007-01-28 07:46:46 · answer #3 · answered by science teacher 7 · 0⤊ 0⤋