A scientist is using an ampicillin-sensiitive strain of bacteria that cannot use lactose because it has a nonfunctional gene in the lac operon. She has to plasmids. One contains a functional copy of the affected gene of the lac operon, and the other contains the gene for ampicillin resistance. Using restriction enzymes and DNA ligase, she forms a recombinant plasmid containing both genes. She then adds a high concentration of the plasmid to a tube off the bacteria in a medium for bacterial growth that contains glucose as thee only energy source. This tube (+) and a control tube (-) with similar bacteria but no plasmid are both incubated under the appropriate conditions for growth and plasmid uptake. The scientist then spreads a sample of each bacterial culture (+ and - ) on each of the three types of plates indicated below.
2007-01-25
10:15:34
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2 answers
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asked by
hersheykiss8908
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Science & Mathematics
➔ Biology