English Deutsch Français Italiano Español Português 繁體中文 Bahasa Indonesia Tiếng Việt ภาษาไทย
All categories

A scientist is using an ampicillin-sensiitive strain of bacteria that cannot use lactose because it has a nonfunctional gene in the lac operon. She has to plasmids. One contains a functional copy of the affected gene of the lac operon, and the other contains the gene for ampicillin resistance. Using restriction enzymes and DNA ligase, she forms a recombinant plasmid containing both genes. She then adds a high concentration of the plasmid to a tube off the bacteria in a medium for bacterial growth that contains glucose as thee only energy source. This tube (+) and a control tube (-) with similar bacteria but no plasmid are both incubated under the appropriate conditions for growth and plasmid uptake. The scientist then spreads a sample of each bacterial culture (+ and - ) on each of the three types of plates indicated below.

2007-01-25 10:15:34 · 2 answers · asked by hersheykiss8908 2 in Science & Mathematics Biology

2 answers

That's the question? Summarize this? Nothing more? I can probably answer you here, but I'm lost as to what you want. Can you throw me a bone here?
Email me @
fortitudinousskeptic@yahoo.com
I can probably help you with this. - kevin

2007-01-25 10:24:39 · answer #1 · answered by Anonymous · 0 0

No, thanks. I get paid to edit.

2007-01-25 10:19:23 · answer #2 · answered by notyou311 7 · 0 1

fedest.com, questions and answers