What really exacly happened during metaphase and anaphase of M phase in interphase of the cell cycle?

Question

We all know that during either mitosis (the production of 2daughter cells with the same diploid state of the parent cell) or meiosis ( the production of daubghter cells with half the chromosomalnuber that of the parent cell) of the cell cycle, the chromosomes before anaphase lined up themselves at the equitorial plane of the cell. At this point what is really going on inbetween centromeres before proanaphase?

Answer 1

Connection checking and stress monitoring. A centromere, or more specifically the kinetochore, cannot be connected to both asters/MTOC and if it is the cell will not continue to anaphase, the mechanism that senses this is through aurora kinases, INCENP, Survivin, and SMC proteins among many other players and is a stress/tension measure. What I can tell you is this is a very active field of investigation, the mechanism I gave you came from a fellow grad students data that was presented about seven months ago and that the whole picture is not known yet. I suggest reading what's below and doing a pubmed search for Arshad Desai, he's an amazing scientist who is actively pursuing this exact question at UCSD, a world-renowned research institution for cell biology. The papers below are his and a good to start, I suggest going through the review first. Best of luck.

Answer 2

The above answer is fine, but also check here for a overview:

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=books&doptcmdl=GenBookHL&term=anaphase+AND+mboc4%5Bbook%5D+AND+374246%5Buid%5D&rid=mboc4.section.3349#3371

Essentially the chromosome alignment acts as a mechanism to reliably separate all chromosomes into 2 daughter cells, and guard against loss of connection to the mitotic spindle.

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=books&doptcmdl=GenBookHL&term=anaphase+AND+mboc4%5Bbook%5D+AND+374246%5Buid%5D&rid=mboc4.section.3349#3371