The alchool precepitation step calls for over night, but I was wondering if it could sit over the weekend.
2007-01-24 08:52:11 · 6 answers · asked by little_kame 2 in Science & Mathematics ➔ Biology
Yep, no problem. It can sit in ethanol indefinitely and be fine
2007-01-24 08:56:48 · answer #1 · answered by floundering penguins 5 · 1⤊ 0⤋
A lot of the talent would either be out of a job or pushed to NXT or OVW. A couple of the current top TNA guys like AJ Styles, Abyss, The Dudley's, Jeff Hardy, Bobby Roode, Kurt Angle and Austin Aries would go after the titles almost straight away or be in the main event. A benefit that might come of this would be a huge revamping of the tag team division though, i mean they'd have a lot of extra talent that needed something to do. The knockouts would fall into nothingness, like most WWE divas nowadays - irrelevant. But as long as Vince is in charge he'd probably bury most of them. And if you're wondering.... No Sting wouldn't be on the roster, he refuses to work for Vince McMahon.
2016-05-24 05:16:08 · answer #2 · answered by Susan 4 · 0⤊ 0⤋
Many times I have done the Ethanol precipitation over the weekend at -20 or -80, that should be no problem at all.
2007-01-24 09:12:15 · answer #3 · answered by btpage0630 5 · 0⤊ 0⤋
In my experience, if I need to let the ppt step sit over the weekend I leave it out at room temp or maybe 4ºC, not 20ºC. Leaving it in the freezer for that amount of time tends to ppt out salts and other stuff you don't want. the warmer temps tend to not have this problem.
I work on plants, though, so this might not be a problem for animal extractions.
2007-01-25 23:48:37 · answer #4 · answered by plantgirl 3 · 0⤊ 0⤋
Yeah, you can leave it over the weekend, but not for a very long time, if you want to do that, spin it down after a day and remove the s/n and allow it to dry.
2007-01-25 13:12:05 · answer #5 · answered by gibbie99 4 · 0⤊ 0⤋
If its ethanol yes, isopropanol no.
2007-01-24 15:50:56 · answer #6 · answered by rgomezam 3 · 0⤊ 0⤋