what is the very best method for BOTH isolating AND EXTRACTING pure DNA from any living sample (fruit, etc.)?

Question

, be it precipitating below a chilled alcohol &/or H2O environment, or a string or ball of relatively dry 100% pure DNA...

AND, is DNA STILL viable & "alive" dry and by itself? if not, can it be revived / recovered, and to what extent?

can the DNA be used to create and grow any life forms, heal/splice with existing life forms, or used in interesting chemical reactions?

what is Qiagen?

is there a "home remedy" available, ie. something close enough to that of large scale labs, but employs household products?

Answer 1

Qiagen columns (or other silica based columns) work well, and are consistant. Other protocols I've used can produce better yields, but rarely cleaner DNA, but in my hands at least they're inconsistant (and take a lot longer).

Qiagen makes purification columns for all sorts of DNA/sources, so there is probably a plant genomic kit available.

Drying DNA doesn't wreck it. When you order primers, they come as dried samples. When you do any number of purification procedures, there is a step where you dry the DNA pellet. Adding water or tris or te to the sample is enough to reconstitute it and make it functional again.

Answer 2

I don't know what the best method is ( i have only used DNeasy kits by Qiagen and the chilled alcohol technique). However dried DNA can be reconsitutied and used for some needs.