what is the polarity in H.P.L.C& G.C Instrumentation?

Answer 1

This question is not totally clear to me. But when we speak about polarity in chromatography often we are referring to how polar the stationary phase may be. For the LC the stationary phase is the surface of the beads on the inside of the column. For GC it is a coating in the inside wall of the capillary.

As the polarity of the stationary phase increases it will retain polar compounds to a greater degree and non-polar compounds will be less retained. So the degree of polarity becomes a simple way to describe how a particular stationary phase will function.

The reason I was confused is because you write instrumentation. These instruments are configurable in a myriad of ways. It all depends on the sample ports, columns (stationary phase), detectors and carriers (mobile phase). So there is no inherent polarity for the instrument. As I wrote polarity is most commonly associated with the stationary phase, secondarily associated with the mobile phase.

Answer 2

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