prepare a table showing the similarities and difference between the light and electron microscope?

Answer 1

Electron microscope: beam of electrons, focused by magnets, image seen on screen or printout, image colorless, much greater magnification.

Light microscope: beam of light, focused by glass lenses, image seen with eyes, image has color, magnification not as great

Answer 2

differences between a light and electron microscope With an ordinary light microscope the average resolution achievable is 300 nm (m x 10-9) with an electron microscope this is increased to 10 nm. The reason the resolution varies so much is that the resolution is calculated by halving the wavelength of the power source in light microscopy this is the wavelength of visible light between 400 and 700 nm. Electrons have a much shorter wavelength of about 0.005 nm. Electron microscopy uses electrons with energies of a few thousand electron volts energies 1000 times greater than visible light. The reason that the average resolution is greater than this number is due to the construction of the microscope it’s self, however the resolution can be pushed to 0.1 nm. the depth of field this is poor on light microscopes, but high on electron microscopes. Also the Field of view between the microscopes varies greatly but, good on both the light and electron. the electron microscope is not suitable for the magnification of living things. A light microscope can be used to look at real living or non-living items. Both types of microscope contain the following objects: Condenser lens (magnetic coil in EM), specimen holder and objective lens (magnetic coil in EM). The electron microscope (EM) contains a projector lens, which is made of magnetic coil. Magnification in the electron microscope can be varied from hundreds to several hundred thousands of times. This is done by varying the strength of the projector lens. This can not be achieved with a light microscope. light microscopes tend to be quite inexpensive, this allows any one interested in microscopy to purchase a light microscope. Electron microscopes however prove to be expensive. Disadvantages of all electron microscopy are that the samples have to be cut incredibly thin (usually with a glass or diamond knife) so the electrons will collide with them. Also there is no guarantee that what goes into the microscope is reality, as to see the objects detail more clearly the objects have to be heavily stained with a metal (usually uranium) and there is no guarantee that this does not damage the samples. If the staining does not damage the sample it is likely that the heavy metal salts used to melt away the electrons will. The main difference between light and electron microscopes is the amount of resolution that can be achieved with electron microscopes to light microscopes. The main similarities are the contents within the microscopes.