You are preparing reagents for your dideoxy sequencing project. You want to make a stock solution of ddGTP (514.93 g/mol). How much ddGTP will you need to weigh out to make 1000µl of a 100mM stock?
a.5.15x10-1 grams
b.5.15x10-3 grams
c.5.15x100 milligrams
d.5.15x104 micrograms
2007-01-14 04:17:07 · 5 answers · asked by Anonymous in Science & Mathematics ➔ Chemistry
You can attack this several ways, but let's use familiar units.
1000uL = 1mL = 0.001 L
100mM = 0.1M
So (0.1M)(0.001L) = 0.0001moles X 514.93 g/mole = 0.0515g
0.0515g = 5.15 x 10^-2 g so answers a,b are out
0.0515g = 51.5mg = 5.15 x 10^1 milligrams so c is out
0.0515g = 51.5mg = 51500ug = 5.15 x 10^4 micrograms Yahoo!
Check my math, I did this pretty fast.
2007-01-14 08:23:36 · answer #1 · answered by Peter Boiter Woods 7 · 2⤊ 0⤋
i think its D bec u need is micrograms since the given is g/mol.
Good Luck ^_^
2007-01-14 04:28:57 · answer #2 · answered by ImMe 1 · 0⤊ 0⤋
b
2007-01-14 04:20:16 · answer #3 · answered by twism 3 · 0⤊ 0⤋
can u be a lil more clear
2007-01-14 04:19:32 · answer #4 · answered by jessica 2 · 0⤊ 1⤋
My GUESS, A
2007-01-14 04:20:43 · answer #5 · answered by Jack P 3 · 0⤊ 0⤋