You are preparing reagents for your dideoxy sequencing project. You want to make a stock solution of ddGTP (514.93 g/mol). How much ddGTP will you need to weigh out to make 1000µl of a 100mM stock?
a.5.15x10-1 grams
b.5.15x10-3 grams
c.5.15x100 milligrams
d.5.15x104 micrograms
2007-01-14 00:32:51 · 3 answers · asked by Anonymous in Science & Mathematics ➔ Chemistry
good question? do not have a clue
2007-01-14 00:43:11 · answer #1 · answered by nj2pa2nc 7 · 0⤊ 0⤋
Its a molarity question (see general chem text).
1. Determine the moles of solute present in 1000µl of a 100mM solution (there's some metric conversion fun here).
2. Convert the moles of solute to mass using molar mass (514.93 g/mol).
2007-01-14 09:01:23 · answer #2 · answered by ChemDoc 3 · 0⤊ 0⤋
be less annoying, and let people know what you are asking about In the title.
2007-01-14 08:52:39 · answer #3 · answered by wizzardx3 2 · 0⤊ 0⤋