what is the effect of pH and temperture on enzyme function?

Answer 1

Most enzymes will operate best at an optimum pH and temperature because outside these ranges the configuration will change and alter the shape of the active site rendering it less efficient or useless.

At high temperatures, the structures can denature causing the enzyme to work less efficiently if at all.
At low temperatures the activity is decreased because particles slow down as the temperature approaches freezing.

At low pH the R groups on the amino acids of the enzymes will become more acidic (NH4+ & COOH) which may disrupt hydrogen bonds and alter the structure.
At high pH the R groups become more alkaline (NH3 & COO-) which can disrupt hydrogen bonds and alter the structure.
The optimum pH will ensure the acidic and alkaline groups are as they should be for the enzyme to work.

n.b. different enzymes will (usually) have different optimum pH and temperatures :)

Answer 2

Well, the optimum tempurature for enzymes to work is 98.6f or 37c. anything above this would denature the enzyme so the enzyme would be unable to function correctly. The same is for tempurature s below, however the enzymes do not denature they just slow down. pH affects it because an acid can denature an enzyme also..xx

Answer 3

first we must know that each enzyme has specific PH & temperature which at them each enzyme becomes at its optimum activity (heighest rate of activity)
1 - temperature : as the temperature increase the reaction of enzyme and substrate increase till the optimum temperature at which the enzyme gives its heighest rate due to the increasing of the kinetic energy of both enzyme and substrate molecules by continue the increasing of that the rate of enzymatic reaction begains to decrease due to the denaturation of the enzyme molecules
2 - PH : as the PH increase the the rate of enzymatic reaction increase till the optimum activity of the enzyme but by continue the increasing of PH the rate of enzymatic reaction begains to decrease because PH may cause preceptation or denaturation to to enzymes so the reaction stopes

Answer 4

extreme ph or extreme temperature can make it function slower or too fast or not at all. the optimum ph and temp can make it function the best.