2007-01-10 02:38:40 · 3 answers · asked by sishkage 1 in Science & Mathematics ➔ Biology
Assuming you are using a steam autoclave, then the two are linked. The only way to get a higher temperature, is by increasing the steam pressure. So, if the instructions are for 15lbs of steam, that will give an operating temperature of 121°C.
Ultimately, it is the high temperature that does the killing.
2007-01-10 03:20:22 · answer #1 · answered by Anonymous · 0⤊ 0⤋
The proper temperature is more important than the air pressure. If the air pressure is lacking, it takes longer to heat up and you may have to increase the autoclaving time, but it is the heat that does the major stirilization.
2007-01-10 02:47:51 · answer #2 · answered by MrKnowItAll 6 · 0⤊ 3⤋
Well, you can't achieve the proper temperature without sufficient air pressure.
However, if you achieve the right pressure but don't bring the temperature up enough, you won't kill everything.
You need both, really, but it is the heat that sterilizes.
2007-01-10 02:42:43 · answer #3 · answered by John V 4 · 1⤊ 1⤋