why use internal standard in gas chromatography?

Question

what source of errors are compensated by using internal standard? and difficulties arise in using an internal standard method of quantitation for essential oil

Answer 1

1) injection technique - didn't get it all into the chamber/sample not right size
2) chamber not warmed up - did not volatilize sample entirely & immediately
3) leaky septum
4) gas tank is running down & flow is slower/weaker than it should be
5) (more for the old bead packed columns than the newer hollow ones) the column is soaking up some of your sample

I can only answer the first part of your question.

;-)

Answer 2

i have no idea.

i'll edit my answer later ok.