Hi there, some time ago I worked on alcohol separations by Carbowax 1500 packed GC using FID and N2 as carrier. The alcohols are methyl, ethyl and 1-butyl. Somebody told me that there's an equation that relates the %mole to the %area, obviously there's an equation for each alcohol. And I'm going mad trying to find them. So the question is: can you tell me the equations, for god's sake?
Please help me...!!!!!!
2007-01-04 18:52:02 · 2 answers · asked by Calculus Teacher 2 in Science & Mathematics ➔ Chemistry
There is no equation that can relate the %analyte to the % area. It is impossible to guess the volume of gas actually being injected after the splitter.
You must create a calibration curve using a set of standards. I usually create a curve on excel, and then plug in my unknown.
The Flame Ionize Detector is a very linear detector, a calibration curve should be straight as an arrow. However, you cannot calculate the total area under all the peaks, and divide them by the area of a single peak. This is a common mistake, each compound has a significantly different detector response, and therefore, each compound must have its own calibration curve built. There is some relation to molecular mass and detector response, but it isn't worth it to figure it out.
2007-01-04 22:22:51 · answer #1 · answered by Stonerscientist 2 · 1⤊ 0⤋
Please give more information. That doesn't make any sense. %mole? What does this mean? However, I can tell you that without a calibration curve, the peak area in GC analysis is pretty useless for determining anything other than column length (which I am sure you already know).
2007-01-04 21:17:07 · answer #2 · answered by Ross P 3 · 0⤊ 0⤋