How do Biologists make use of Restriction Enzymes?

Question

i know what restriction enzymes are but i dont know how they use them

Answer 1

biologists use them to cut and paste pieces of DNA apart and together

they can also be used to cut up DNA into strands of varying lengths to characterize samples

Answer 2

Since you already know that restriction enzymes cut DNA at specific sites, I'll focus on the uses. Scientists have a difficult time working with large pieces of DNA. This makes it difficult to work with the DNA of most organisms, who have millions of base pairs of DNA. We need a tool to fragment the DNA so we can separate out the pieces of DNA that we are most interested in sequencing or cloning (in the DNA sense, not in the Dolly the sheep sense). Restriction enzymes were the first way scientists could cut DNA into managable pieces.

Answer 3

Let's see, if I remember correctly we used restriction enzymes in chemistry/biology to stop reactions at certain points in time so that we could see what the effects of something at certain points.

For example, we wanted to test the pH of a reaction at 10 seconds, 30 seconds, a minute, etc. At each interval, we would take a sample of the reacting chemicals and throw in a restriction enzyme of sorts to stop the reaction. That way we could get accurate titration results.

Answer 4

See the 'Restriction enzymes as tools' section of this article.

Answer 5

They cleave the DNA at specific gene sequences.
Scientists use this in genetic engineering.