What is the expected molecular weights of non-reduced and reduced IgG?

Question

What is the expected molecular weights of non-reduced and reduced IgG and the reason
for this relating to the heavy and light chains?

The Expected Molecular weight obtained from SDS-PAGE.
using 7.5% gel for non-reduced sample and 12.5% gel for reduced sample

Answer 1

IgG heavy and light chains are held together by disulfide bonds. reduced form is disufide bonded chains while oxidized form would result in 2 or 4 chains...hence separation using less concentrated gel.

Google the masses