What is spectrofluorometry?

Answer 1

To simplify the first answer, basically it's a technique measuring the amount of light something gives off (fluorescence). Often amino acids like tryptophan give off light when excited, so spectrofluorometry can be used to, say, measure how many tryptophans are present or to monitor the activity of a protein with tryptophan in it.

Answer 2

The excitation and emission spectra can be measured with a fluorometer (sometimes called spectrofluorometer) It records the excitation and emission spectra of the fluorescing sample, which represent the relative photon intensity per wavelength interval. The light, emitted from a xenon lamp, is directed onto a monochromator grating. The grating splits the light up into its rainbow colors. The slits allow only a narrow band of light to pass through. This band of approximately monochromatic light is then focused onto the sample. Light emitted from the sample is collimated and directed into the emission monochromator. Built similar to the excitation monochromator, it lets only a narrow band of wavelengths to pass. A photomultiplier collects the light and measures its intensity.

Answer 3

Fluorescence spectroscopy or fluorometry or spectrofluorimetry is a type of electromagnetic spectroscopy used for analyzing fluorescent spectra. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light of a lower energy, typically, but not necessarily, visible light. A complementary technique is absorption spectroscopy.

Answer 4

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What is spectrofluorometry?

Answer 5

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