i'm doing the experiment to isolate the azotobacter,
refer Berjey's manual...
said that the azotobacter is fluorescent pigment...
can anyone told me how to test the bacteria that i was isolated is fluorescent ....???????
need what chemical or equipment ?????
2006-12-25 23:30:48 · 1 answers · asked by joli3133 1 in Science & Mathematics ➔ Biology
Acetobacter can be easily distinguished in the laboratory by their growth of colonies on a medium containing about 7% ethanol, and enough calcium carbonate to render the medium partially opaque. When Acetobacter colonies form enough acetic acid from the ethanol, the calcium carbonate around the colonies dissolves, forming a very distinct clear zone.
in order to isolate by using its fluoresecent keep a photographic plate so the we can know the position of the azotobacter in the plate using the immpression of its flourescence on the plate.
2006-12-26 02:49:57 · answer #1 · answered by Dinesh D 1 · 0⤊ 0⤋