D o you have idea about Dornase alfa, Pulmozyme Inhalation Solution?

Question

hello,

if you have any idea about how the drug is isolated, purified, solubilization and stabilization of Pulmozyme Inhalation Solution.

thank you

Answer 1

Some nebulizer equipment is administered at the doctors.
This is done with a machine that can be trained to the use
by patient. The treatment is for cycstic fibrosis, some throat
problems, some breathing problems, with an inhaler. The
general treatment is when due growth of the lung is not
remission accurate, meaning there is a lung block or problem
that doctors cannot remove perhaps congestion, or infection.
Any lung inflamation must be treated with constant update.
The types of drug solutions are tested for diameter capillatia
to be sure there is no swelling, or poison protective coating
from nearby organs, or fresh tissue. Generally the treatment
is for short periods to keep constant updates in the event of
unremovable problems that need be dissolved or dislodged.
The success ratio of recent solutions is 20% higher than in
the 1960 decade now more typical of 85-95%.

Answer 2

Pulmozyme (dornase alfa) Inhalation Solution is a sterile, clear, colorless, highly purified solution of recombinant human deoxyribonuclease I (rhDNase), an enzyme which selectively cleaves DNA. The protein is produced by genetically engineered Chinese Hamster Ovary (CHO) cells containing DNA encoding for the native human protein, deoxyribonuclease I (DNase). Fermentation is carried out in a nutrient medium containing the antibiotic genta-micin, 100–200 mg/L. However, the presence of the antibiotic is not detectable in the final product. The product is purified by tangential flow filtration and column chromatography. The purified glycoprotein contains 260 amino acids with an approximate molecular weight of 37,000 daltons (1). The primary amino acid sequence is identical to that of the native human enzyme.

Pulmozyme is administered by inhalation of an aerosol mist produced by a compressed air driven nebulizer system. Each Pulmozyme single-use ampule will deliver 2.5 mL of the solution to the nebulizer bowl. The aqueous solution contains 1.0 mg/mL dornase alfa, 0.15 mg/mL calcium chloride dihydrate and 8.77 mg/mL sodium chloride. The solution contains no preservative. The nominal pH of the solution is 6.3.

Additional information on the chemisrty and pharmacology of Pulmozyme can be found at:
http://www.rxlist.com/