what exactly is the process of cloning?

Answer 1

Isolate donor nucleus
Get unfertilized eggs
Remove the egg's nucleus
Insert donor nucleus
Place the egg into womb

1. Isolate donor nucleus
Isolate the nucleus from a somatic (non-reproductive) cell of a adult donor sheep. The nucleus contains the complete genetic material of the organism. This step is repeated many times to gather many cell nuclei. A very small needle and syringe (suction device) is used to poke through the cell membrane to capture the nucleus and remove it from the cell.


2.Get unfertilized eggs
Retrieve unfertilized egg cells (reproductive) from a female sheep. Many eggs are needed since not all of them will survive the various steps of cloning.


3. Remove the egg's nucleus
Remove the egg cell's nucleus, which contains only one-half of the sheep's genetic material. A very small needle and syringe (suction device) is used to poke through the cell membrane to capture the nucleus and remove it from the cell.


4. Insert donor nucleus
Insert the nucleus, with its complete genetic material, isolated from the donor mammal in Step 1 into the egg cell that has no nuclear material. The egg's genetic material now contains all traits from the donor adult. This egg is genetically identical to the donor adult.


5. Place the egg into womb
Place the egg into a female sheep's womb. Only a small percentage of eggs placed in the womb will start to mature. Those eggs that survive will continue to develop into embryos. The egg matures in the womb. When the offspring is born, it is a clone (genetically identical) of the donor sheep.

Answer 2

DNA cloning is a technique that is used widely to produce proteins such as insulin for diabetics. It is a common misunderstanding that cloning means producing a whole animal or worse producing a human from a single cell.
To clone insulin or the enzymes that we find in washing powder we extract and identify the gene of interest. This is then inserted into a plasmid (a circular chromosome found in bacteria and yeasts) which is then taken up by a colony of bacteria or yeast which then go through many cycles of replication producing both the gene and the protein product of the gene in vast quantities. This is purified and hey presto - human insulin instead of the pig insulin extracted from porcine pancreases that made life misery for diabetics.

Answer 3

Cloning a cell means to derive a (clonal) population of cells from a single cell. This is an important in vitro procedure when the expansion of a single cell with certain characteristics is desired, for example in the production of gene-targeted ES cells. A valuable tissue culture technique used to clone distinct lineages of cell lines involves the use of cloning rings (cylinders). According to this technique, a single-cell suspension of cells which have been exposed to a mutagenic agent or drug used to drive selection is plated at high dilution to create isolated colonies; each arising from a single and potentially clonally distinct cell. At an early growth stage when colonies consist of only a few of cells, sterile polystyrene rings (cloning rings), which have been dipped in grease are placed over an individual colony and a small amount of trypsin is added. Cloned cells are collected from inside the ring and transferred to a new vessel for further growth.

Most individuals began as a single cell and are therefore the result of clonal expansion in vivo

Answer 4

A cloned cow is in basic terms a cow that develop into no longer produced by organic duplicate technique, e.g. no sperm and egg turning out to be a member of to make a zygote. what's comprehensive is that DNA from yet another cow is taken and inserted right into a fertilized egg changing the pre-present DNA there. So the cloned cow's DNA is precisely the comparable as its "determine", and it has no mama cow nor papa cow. Its intercourse of path may be the comparable as its determine. The FDA has got here across no distinction between meat from cloned cows and meat from standard cows. with the aid of fact of this they're pondering approving it for intake. of path, they're nonetheless a strategies far off from that approval as ethical reasons may additionally come into play. i do no longer understand why it is easy to opt to clone cows or pigs for intake. it variety of feels to be a greater costly technique than the traditional breeding technique. innovations you, i'm no longer a rancher so i does not understand the fees or the approach that they do those days. according to danger some companies do in vitro fertilization now to decrease injury to cows. And in the event that they're, according to danger, cloning could be a assure that the animals they have won't have any genetic sickness.

Answer 5

someone above said that the chances of cloning is like 0.12%. It may be true but with the technology booming, it's bound to increase like nothing

Answer 6

A nucleus from an egg cell is taken and placed in an enucleated somatic cell and then zapped with electricity. There is something like a 0.12% chance of success.

Answer 7

Putting on a silly wig and a red nose

Answer 8

http://www.channel4.com/science/microsites/S/science/medicine/cloning_faqs.html - This is the link that I was looking at yesterday, if you visit it you will find it explains it all. Hope this is useful and what you needed. Happy New Year!

Answer 9

exactly here:
http://en.wikipedia.org/wiki/Cloning

Answer 10

it's not really exact.