What is the function of a restriction maps?

Answer 1

To insert a gene into a vector, you need restriction maps because you want to know where you should insert the gene in. What are the available cut sites around and which one should you use.

Take for example ECoRI as the restriction site, if you do not have the restriction map, you do not know whether the vector has an ECoRI cut site, the addition of the cut site in your Gene of Interest may become useless. Also, is the after the cut site? Do you have other coactivators to help in transcription or some other sites would be better? There may be a HinDIII cut site around and it serves as a better restriction site for your gene of interest. You may now want to add a HinDIII cut site instead of an ECoRI.

A restriction map allows the technologist to clearly choose the best site and add the required restriction site on the gene of interest.

Answer 2

Restriction Mapper

Answer 3

A new DNA molecule is like uncharted territory to a scientist. Like any explorer, the first thing a scientist does with new territory is to map its important features, such as size and the relative locations of certain parts. Scientists have a unique set of tools to map new DNA. Maps of DNA are called restriction maps, named for the enzymes used as mapping tools.

Restriction enzymes are proteins produced by bacteria. They act as a very primitive type of bacterial immune system. When a bacterial cell is invaded by foreign DNA (say, from a virus), restriction enzymes act as DNA scissors, cutting the foreign DNA into pieces so it cannot function.

Restriction enzymes recognize and cut DNA at specific sequences of nucleotides (called restriction sites), and the sizes of the DNA fragments correspond to the distances (in base pairs) between restriction sites. Every time the same piece of DNA is cut with a given enzyme, the same fragments are produced
Working out the locations of the different restriction sites is a problem in logic. Doing a restriction enzyme digest with a single enzyme, for example, only tells you how many sites are present for that enzyme. Doing restriction digests with more than one enzyme at a time can give clues as to where those restriction sites are in relation to each other

Answer 4

To let researchers keep track of where on a particular piece of DNA different restriction enzymes cut. If you are trying to clone a gene into a plasmid, you need to know where to cut both the gene and vector, and what endonucleases to use.

Answer 5

you just substitute the x variables with y and isolate y. I think the only thing that would make it wrong is if the denominator becomes 0. :D

Answer 6

veerbehad's answer is one of the best I have seen here in a long time. good job!