hey thanks,
just curious how you would test for a lipid, using a reagent possibly??
and the colour change..
for example.. to test for protein, you use biurets reagent, and it changes from colourless to purple..
thats the sort of answer i would like
thanks :)
2006-12-13 04:20:25 · 10 answers · asked by sir_pete_the_great_one 2 in Science & Mathematics ➔ Biology
In most high schools, the test for lipids is the "brown paper test", where you press the substance against brown paper towels and see if it turns dark and translucent.
2006-12-13 04:29:14 · answer #1 · answered by Kerahna 3 · 1⤊ 0⤋
Lipid Paper Test
2016-12-18 14:07:09 · answer #2 · answered by ? 4 · 0⤊ 0⤋
As lipids don't dissolve in water solvents are needed. The emulsification test for lipids is generally used. Ethanol is added to the sample and you shake it so that the lipid dissolves. You then pour the mixture into water. If the mixture or sample contained lipid a white emulsion will be produced.
2006-12-13 05:28:31 · answer #3 · answered by Anonymous · 1⤊ 0⤋
the easiest way to check for lipids is to use brown paper.
put a small amount of the lipid on brown paper and leave it to dry.
the spot will become translucent if lipids are present.
don't put too much solution on the paper because it takes ages to dry
2006-12-13 05:43:49 · answer #4 · answered by polly 3 · 0⤊ 0⤋
1.all cells come from pre-existing cells 2.the microscope was invented 3cells, tissues, organs, organ systems, organisms 4regulate what enters and leaves the cell 5cell wall 6mitochondria 7contain the DNA 8break down waste products 9door 10 i think molecules move from an area of higher concentration to lower 11 it's either active transport or facilitated diffusion 12prokaryotes 13chloroplasts, cell wall 14 ribosome 15their function 16 17 i think spontaneous generation 18endoplasmic reticulum 19Golgi apparatus 20impermeable hope that helps, i'm good at art too. bio isn't as bad as earth science i have to admit.
2016-03-29 05:48:38 · answer #5 · answered by ? 4 · 0⤊ 1⤋
Lipids include steroids as well as fats and oils in formal classification. This next test is only for triglyceride fats or oils.
Add a small amount of suspected lipid to 1 gram solid potassium bisulfate, KHSO4 in a test tube. Heat over a burner flame. When fumes start rising in the test tube, wave your hand over the top to waft just a little bit of the vapor toward you to smell it. The acrid odor of acrolein is positive for fat or oil lipid. Try it once on butter to familiarize yourself with the odor. Acrolein is a "tear gas," by the way, so you won't want much!
The H+ of KHSO4 plus the high temperature of heating fat with the solid salt hydrolyzes fats and oils to glycerol and fatty acids. The treatment further dehydrates glycerol to acrolein:
HO-CH2-CHOH-CH2-OH + H+ ===> CH2=CH-CH=O
2006-12-13 04:32:55 · answer #6 · answered by steve_geo1 7 · 1⤊ 1⤋
You could also stain with Oil Red O or a Sudan dye if you have sections. However, these stains only work on frozen sections- if they have been paraffin embedded you will lose staining as the ethanol will extract the lipid.
2006-12-13 06:04:17 · answer #7 · answered by Anonymous · 1⤊ 0⤋
Definetly, as a science teacher, I use the emulsion test outlined above with the students. It's simple, fast and accurate qualitative as opposed to quantitative assessment.
2006-12-13 11:38:10 · answer #8 · answered by tigress_taz 2 · 0⤊ 0⤋
For the best answers, search on this site https://shorturl.im/axhR3
C A B D B C A B D D B A B A C B D A A B
2016-04-07 03:01:48 · answer #9 · answered by ? 4 · 0⤊ 1⤋
Yell out, "Are there any lipids here?"
2006-12-13 04:32:51 · answer #10 · answered by Anonymous · 0⤊ 3⤋