could anybody teach me how to extract DNA from bone using the traditional method?

Question

starting from the prosess of sample preparation till the extraction method

Answer 1

This is a link to some different techniques for extraction for bone as well as other materials.

http://www.uga.edu/srel/DNA_Lab/OldDNA99.rtf

Answer 2

Extraction of DNA from Bone

1. Pulverize up to 2g bone observing appropriate precautions to avoid contamination and excess heat. The amount of bone used initially will depend on the quality of bone sample. Avoid the outer surface of bone, especially if mitochondrial DNA is being examined, in order to reduce contamination.

2. Place the bone in up to 3ml of freshly prepared Proteinase K Digestion Solution in a 15ml tube and incubate at 56°C for 1 hour (see Notes 1–2).

Notes:
1. Coarsely ground bone may require longer incubation times.
2. Add Proteinase K Digestion Solution to cover the entire sample.

3. Remove the remaining bone by centrifugation at 5000rpm for 5 minutes. Transfer solution to a new 15ml tube.

4. Add 2 volumes of DNA IQ™ Lysis Buffer to the solution.

5. Vortex the DNA IQ™ Resin for 10 seconds at high speed or until resin is thoroughly resuspended. Add 15μl of resin to the sample. Keep the resin resuspended in the stock bottle while dispensing to obtain uniform results.

6. Vortex the sample/Lysis Buffer/resin mixture for 5 seconds at high speed.

7. Incubate at room temperature for 10 minutes, mixing one to three times by inverting the tube.

8. Vortex for 5 seconds at high speed, and place the tube in the Magnetic Stand. Carefully remove the solution. If resin does not form a distinct pellet on the side of the tube, vortex the tube and quickly place back in the stand.

9. Add 100μl of Lysis Buffer, vortex for 2 seconds at high speed. Carefully transfer the mixture to a 1.5ml tube, making sure all of the resin has been transferred.

10. Vortex for 2 seconds at high speed, place the tube in the Magnetic Stand, and carefully remove and discard the solution.

11. Add 100μl of prepared 1X Wash Buffer (see DNA IQ™ System—Small Sample Casework Protocol Technical Bulletin #TB296). Remove tube from the Magnetic Stand and vortex for 2 seconds at high speed.

12. Return tube to the Magnetic Stand. Dispose of all Wash Buffer.

13. Repeat Steps 11 and 12 two more times for a total of three washes, making sure all of the solution has been removed after the last wash.

14. With the tubes in the Magnetic Stand and the lids open, air-dry the resin for 5 minutes.

Note: Do not dry for more than 20 minutes, as this may inhibit removal of DNA from the resin.

15. Add 25–100μl of Elution Buffer, depending on how much biological material was used. Lower elution volume ensures a higher final concentration of DNA.

16. Close the lid, vortex tube for 2 seconds at high speed. Place at 65°C for 5 minutes.

17. Remove tube from the heat source, and vortex for 2 seconds at high speed. Immediately place in the Magnetic Stand.

18. Transfer the solution to a container of choice.

Note: The DNA solution can be stored at 4°C for short-term storage or at –20°C or –70°C for long-term storage.