i done an experiment where by i extracted the dna from a kiwi. Can anyone help me with this question.....
What is the main contaminant of dna prepared in this way and how could it be removed??? No links please, answers only
2006-12-08 00:19:30 · 6 answers · asked by rachel c 2 in Science & Mathematics ➔ Biology
Not really knowing what techniques you used or even the source (kiwi fruit or kiwi the small flightless bird and if the latter what tissue? blood?) I can only give you very generalised answers. Carry over of protein material may be of concern especially as the proteins may degrade your DNA, however a double phenol chloroform extraction should have put paid to the proteins, at the cost of providing another source of contamination: phenol. Usually you won't have much problem with carrying over phenol if you take care when pipetting the aqueous phase. Potential salt contamination can be dealt with by dialysis against a large volume of TE for around 48 hrs at 4 C. The EDTA in the standard resuspension buffer (TE) is a chelating agent which helps to reducing problems due to some enzymatic action by depriving them of any metal co-factors they may require.
2006-12-08 02:04:45 · answer #1 · answered by Bill T 2 · 0⤊ 0⤋
how the hell did you extract DNA from a kiwi? With access to that kind of technology you should really know the answers to your question. Is this what science has come to!?!
2006-12-08 00:23:24 · answer #2 · answered by think outside the box 2 · 0⤊ 0⤋
What is the main contaminant of dna prepared in this way and how could it be removed???
Prepared in WHAT way, you did not specify method or lab conditions?
2006-12-08 00:23:17 · answer #3 · answered by Stevie t 3 · 0⤊ 0⤋
depends on what method of DNA extraction you have used.
perhaps proteins:
solutions can be made to precipitate proteins out of solution and they can be isolated.
or
salt contamination:
salts can be removed from DNA preparations by washing with alcohol (ethanol)
2006-12-08 00:24:16 · answer #4 · answered by well_clever_i_am 3 · 0⤊ 0⤋
Nice try but you will need to actually read your text book or go to the library and find the appropriate book.
2006-12-08 00:22:19 · answer #5 · answered by KD 5 · 1⤊ 0⤋
Are you in New Zealand ?
2006-12-08 00:26:29 · answer #6 · answered by Scotty 7 · 0⤊ 0⤋