How to calculate the volume of the eluent that i need to put inside my column chromatography?

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How to calculate the volume of the eluent that i need to put inside my column chromatography?

2006-12-06 13:45:58 · 2 answers · asked by rosy blue 1 in Science & Mathematics ➔ Chemistry

2 answers

There is no hard and fast rule on this. It is very dependent of the column material, its pretreatment, type of column you are running (flash, gravity), and need for column stabilization. If this is from your schoolwork, your text must have given a rule of thumb. You will need to find it.

2006-12-09 16:05:32 · answer #1 · answered by Peter Boiter Woods 7 · 0⤊ 0⤋

What is the substance you are analyzing for and what kind of chromatography are you doing?

2006-12-06 22:33:19 · answer #2 · answered by shadowsandfog 2 · 0⤊ 0⤋