You suspect that some of these mutations are lacIS (superrepressor) mutations while others are lacP mutants that prevent the binding of RNA polymerase to the promoter. Describe what haploid and partial diploid genotypes will allow you to distinguish between the lacIS and lacP classes of uninducible mutations.
2006-12-04 04:04:56 · 1 answers · asked by geneticsgirl 1 in Science & Mathematics ➔ Biology
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Grow the mutant and some wild type bacteria with and without an inducer like IPTG or lactose and then do a beta-gal assay
LacIS mutants will have a much lower beta-gal acivity than wt under all conditions. By doing a beta-gal activity vs inducer concentration curve you can find out how much stronger is the effect of the mutant repressor.
LacIR will have the same amount of beta-gal activity in both cases and it will be approximately the same as that for wild type bacteria in the presence of inducer.
Why do you want to complicate things?
2006-12-04 04:27:02 · answer #1 · answered by bellerophon 6 · 0⤊ 0⤋