Plasmid vector that can express cloned cDNA?

Question

can a new plasmid vector be really generated to express cloned cDNA anyone daring a good ans I will give him or her full rating but it should be a good one?

Answer 1

Plasmid vectors are small circular molecules of double stranded DNA derived from natural plasmids that occur in bacterial cells. A piece of DNA can be inserted into a plasmid if both the circular plasmid and the source of DNA have recognition sites for the same restriction endonuclease.

The plasmid and the foreign DNA are cut by this restriction endonuclease (EcoRI in this example) producing intermediates with sticky and complementary ends. Those two intermediates recombine by base-pairing and are linked by the action of DNA ligase. A new plasmid containing the foreign DNA as an insert is obtained. A few mismatches occur, producing an undesirable recombinant.

http://www.accessexcellence.org/RC/VL/GG/inserting.html

Answer 2

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