enzyme activity...plz help?

Question

What are the factors that affect enzyme activity (eg temp, pH)?

Answer 1

The factors that affect enzyme activity are:pH, temperature, substrate concentration and even enzyme concentration, and inhibitors.
There a particular pH at the which the enzyme activity is maximum. This is called the pH optimum of the enzyme.A graph of pH versus enzyme activity shows a bell shape curve.
There is a temperature at which the enzyme activity is 100%. If the temperature is increased by 10 degrees Celsius then the enzyme activity becomes doubled. This is called Q10 of the enzyme. Further increase in the temperature lowers enzyme acivity and at higher temperature the activity is lowered further to become nil.
Substrate concentration is also important. At lower substrate concentration the enzyme activity is dependent on the substrate but at higher substrate concentration it becomes independent of substrate concentration.
At a very low enzyme concentration the enzymes suffer a kind of dilution and become inactivated.
There are substances that are called inhibitors.They impede enzyme or block enzyme activity.A competitive inhibitor binds the active site to block enzyme activity. A non-competitive inhibitor binds to enzyme at some other place to cause change in the three-dimensional structure of the enzyme in such a way that the configuration of the active site is changed and in this case the substrate won't fit in nicely to ensure catalysis.

Answer 2

Exactly those, temperature, pH, also allosterism, substrate availabliity. Also, many enzymes are turned on and off by phosphorylation (sometimes phosphorylation activates and enzyme, sometimes it deactivates an enzyme). Some enzymes are secreted as zymogens (an enzyme in an INACTIVE form), and need to be proteolytically cleaved to become activated (e.g. pepsinogen >>> pepsin)

Answer 3

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Answer 4

temperature, pH, inhibitors, coenzymes.

Concentration of reactants will affect rate of reaction when [R]>[E]; enzymes are saturated.