give a detailed accont on the genetic system of yeast.?

Question

eloborated description of the genetic system of yeast.

Answer 1

We have developed yeast as an expression and genetic system for functional studies of the insulin-degrading enzyme (IDE), which cleaves and inactivates certain small peptide molecules, including insulin and the neurotoxic A peptide. We show that heterologously expressed rat IDE is enzymatically active, as judged by the ability of IDE-containing yeast extracts to cleave insulin in vitro. We also show that IDE can promote the in vivo production of the yeast a-factor mating pheromone, a function normally attributed to the yeast enzymes Axl1p and Ste23p. However, IDE cannot substitute for the function of Axl1p in promoting haploid axial budding and repressing haploid invasive growth, activities that require an uncharacterized activity of Axl1p. Particulate fractions enriched for Axl1p or Ste23p are incapable of cleaving insulin, suggesting that the functional conservation of these enzymes may not be bidirectionally conserved. We have made practical use of our genetic system to confirm that residues composing the extended zinc metalloprotease motif of M16A family enzymes are required for the enzymatic activity of IDE, Ste23p, and Axl1p. We have determined that IDE and Axl1p both require an intact C terminus for optimal activity. We expect that the tractable genetic system that we have developed will be useful for investigating the enzymatic and structure/function properties of IDE and possibly for the identification of novel IDE alleles having altered substrate specificity

Answer 2

they are asserting it quite is an analogous clothier making use of his previous components. no longer very imaginitive. besides the fact that S. cerevisiae's genome is nowhere close to as on the brink of ours as any animals, or maybe then all mammals are nearer than others and all primates are the closest. weird and wonderful how that development works, rather because of the fact the noncoding DNA that's no longer under decision follows it too.... Edit: And to get technical, genetic code =/= genome. people and this species of yeast (and with some exceptions each little thing else in the international) have an comparable genetic code, the three letter designation for each amino acid. The genome is the nucleotide sequence of an organism's DNA. that's what the paper is speaking approximately.

Answer 3

Well, Im not quite sure what the true answer is to your question, BUT, I can tell you I have lived with intestinal yeast overgrowth for 5 yrs. It took over 4 yrs to diagnose it. Its something both men and women can get. Its caused from too many antibiotics and /or a high sugar diet. Which I had done both.

It ate up all the good bacteria in my digestive system and the yeast thats already in our intestines took over.

It poisens the system, if not treated, your organs will begin to shut down. It will screw up everything in your body. It causes many symptoms.

The only thing they really know about the yeast in our systems is...it is used in decomposing of the body.

I have a whole lot of info on yeast, since I have lived with it, I have researched it to death.

Answer 4

A yeast genetic system for selecting small molecule inhibitors of protein-protein interactions in nanodroplets


Cellular processes are mediated by complex networks of molecular interactions. Dissection of their role most commonly is achieved by using genetic mutations that alter, for example, protein-protein interactions. Small molecules that accomplish the same result would provide a powerful complement to the genetic approach, but it generally is believed that such molecules are rare. There are several natural products, however, that illustrate the feasibility of this approach. Split-pool synthesis now provides a simple mechanical means to prepare vast numbers of complex, even natural product-like, molecules individually attached to cell-sized polymer beads. Here, we describe a genetic system compatible with split-pool synthesis that allows the detection of cell-permeable, small molecule inhibitors of protein-protein interactions in 100- to 200-nl cell culture droplets, prepared by a recently described technique that arrays large numbers of such droplets. These "nanodroplets" contain defined media, cells, and one or more beads containing 100 pmol of a photoreleasable small molecule and a controlled number of cells. The engineered Saccharomyces cerevisiae cells used in this study express two interacting proteins after induction with galactose whose interaction results in cell death in the presence of 5-fluoroorotic acid (inducible reverse two-hybrid assay). Disruption of the interaction by a small molecule allows growth, and the small molecule can be introduced into the system hours before induction of the toxic interaction. We demonstrate that the interaction between the activin receptor R1 and the immunophilin protein FKBP12 can be disrupted by the small molecule FK506 at nanomolar concentrations in nanodroplets. This system should provide a general method for selecting cell-permeable ligands that can be used to study the relevance of protein-protein interactions in living cells or organisms.

Answer 5

Yeast gets "puffy" when warm and fresh.
Is this description elaborate enough for you?

Answer 6

http://dbb.urmc.rochester.edu/labs/sherman_f/startedyeast.pdf

Answer 7

Which account you want ? I have ICICI and HDFC account.