a student collects a few leaves to find if chlorophyll a and chlorophyll b are present. what are the step wise procedure for the student to follow? what are the different type of apparatus to be used? what are the safety precautions that should be taken during the experiment? what is the principle involved in this experiment?what are the results?
2006-11-25 01:16:19 · 3 answers · asked by Anonymous in Science & Mathematics ➔ Chemistry
just fyi i would have made this a biology question but anyway, chlorophyll a and b would be present in both leaves its not one or the other. this sounds like homework don't you think that you should be figuring this out on your own. i'll give you a couple hints. you need a solute so that you can separate the pigments. the pigments will travel farther if the are less soluble. Anyway thats all i'm giving you good luck.
2006-11-25 01:56:17 · answer #1 · answered by jrtootsiepop 3 · 0⤊ 0⤋
Dry and crush the leaves using a mortar and pestle.
Add ethanol or isopropyl alcohol to the powdered leaves to extract the pigment.
Us paper chromatography to separate the two forms of chlorophyll. (read up on chromatography)
Measure distance traveled by the two colors of chlorophyll. (see your book for more information)
apparatus: mortar and pestle, beaker, filter paper
Safety: ethanol and isopropyl alcohol are flammable. Do not inhale vapors, keep from flames, wash hands with lots of water.
2006-11-25 01:57:16 · answer #2 · answered by physandchemteach 7 · 0⤊ 0⤋
Use paper chromatography. take test tubes and some leaf samples.Crush the leaves up and put them in the test tubes.Add acetone acid to each of the test tubes.For safety precautions, just wear goggles on your eyes cause' acetone can burn bad!
2006-11-25 02:02:38 · answer #3 · answered by Anonymous · 0⤊ 0⤋