what equipment is used in studying RNAi?
2006-11-22 18:09:16 · 4 answers · asked by Ria 1 in Science & Mathematics ➔ Biology
nice.. but can you be a little more specific and give me more?
2006-11-22 18:13:56 · update #1
electron or ultraviolet microscope
2006-11-22 18:14:24 · answer #1 · answered by Roxy L 3 · 0⤊ 0⤋
RNAi is the natural process whereby an organism can suppress its own mRNA transcriptions' translation by forcing anti-sense RNA onto it, blocking the coding mRNA. It is also done by researchers to purposefully "knockdown" a gene to see what happens next.
Generally, for RNAi, the sample is treated with the RNAi antisense sequence, and then the presence of a "downstream" protein is analyzed.
The sample to be treated is often a flask-based cell cuture. That's the only one I've seen anyways.
That can be done by Western blotting (to get a "yes/no" answer on the expression of a protein), by ELISA (a method with great precision, though fumbly accuracy).
2006-11-23 02:16:36 · answer #2 · answered by indigojerk 3 · 0⤊ 0⤋
recambinant dna technology
2006-11-23 04:17:46 · answer #3 · answered by Anonymous · 0⤊ 0⤋
really strong microscopes
2006-11-23 02:10:32 · answer #4 · answered by Anonymous · 0⤊ 0⤋