Stationary phase and mobile phase are terms in High Pressure Liquid Chromatography.
2006-11-20 21:24:10 · 4 answers · asked by Anonymous in Science & Mathematics ➔ Chemistry
sorry no idea, plz explain ur question
2006-11-20 21:43:43 · answer #1 · answered by Anonymous · 0⤊ 2⤋
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What is stationary phase and mobile phase in Chromatography?
Stationary phase and mobile phase are terms in High Pressure Liquid Chromatography.
2015-08-05 23:12:43 · answer #2 · answered by Anonymous · 0⤊ 0⤋
These are the two phases involved in chromatography. The mobile phase is usually liquid and is the solvent between the two. The solvent can attract some substances that has an affinity to it, thereby separating the polar from the non polar substances. On the other hand, the stationary phase is the media or medium which is a solid substance in which the unknown sample or the solution to be analysed is passed. As the term implies , it is stationary and substances in the unknown sample that has affinity to it or to the reagents in it will not travel along the column, but stay with the stationary medium, thereby allowing a separation of the substances present in the unknown sample/solution.
2006-11-21 07:28:54 · answer #3 · answered by ? 7 · 3⤊ 0⤋
HPLC injects a sample into a mobile (liquid) phase, which is then passed through a column containing the stationary (solid) phase. The liquid phase is your solvent and the medium in which your sample travels. The stationary phase is something in the column that the sample in solution may or may not react with and cause slower elution times.
Here's some examples of stationary phase media at the below link.
2006-11-20 23:06:01 · answer #4 · answered by Arashi K 2 · 1⤊ 0⤋
Chromatography Definition
2016-10-03 03:45:04 · answer #5 · answered by matlock 4 · 0⤊ 0⤋
what is impact ionization?
2013-11-08 04:31:37 · answer #6 · answered by Shalom 1 · 0⤊ 1⤋