To be honest with you i don't quite understand your question, it is often that restriction enzymes are used to create genomic library for which you look into the overlapping sequence and then be able to determine which fragment is next to which. If that is what you have done, you should have used the fragmented DNA sequence to determine the orginall DNA sequence that you digested it with the enzyme, to confirm the sequence you ahve found is correct, you can use PCR, you got the hypothetical sequence, thus by making primers from the start and the end of the sequence (doesn't matter which way round) you should have amplify the same sequence which you started with, you got the amplified DNA (same as the orginal DNA because you used primers that complemented the very start and the very end of the sequence which you found out after the restruction enzyme mapping) run it on gel electrophoresis with a DNA size marker that will include the size of DNA you have sequenced, if the amplified DNA size is the same as the sequence you have found from restriction mapping, that means your primers did complemented from the start and the end, that means the sequence you thought is actually the correct sequnece.
I am sorry, i don't think i am making this clear enough, but anyway, that's the best i can think of.
2006-11-18 09:42:27
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answer #1
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answered by lippy19850528 3
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Your question is not very clear. If you want you can contact me with the specifics.
What type and size of DNA are we talking about (a plasmid, genomic)?
Where did you get the map from in the first place?
How much info do you have on the sequence of the DNA?
The first thing that comes to my mind is to try restricting with different combinations of the enzymes and see (by agarose electrophoresis) if the number and size of the resulting fragments matches the ones that would correspond to the given map.
2006-11-18 12:28:11
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answer #2
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answered by bellerophon 6
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You could try gel electrophoresis. This shows how many fragments the restriction enzymes will cut the DNA into.
2006-11-18 09:07:41
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answer #3
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answered by julezw 2
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hlm124. You answer reads as in case you chosen words at random from the index of a biology text cloth. I have not have been given any theory what biology "A" is yet while this is previous what we interior the states deliberate to be freshman biology (9th grade) then you definately ought to be ashamed. no longer of your lack of understanding, yet of your posturing. In technology there is not any longer something worse than a noisy voice who lacks the integrity to maintain quiet while he/she dosn't be conscious of. Shouting the incorrect answer impacts people better than the main intently whispered certainty. this could be a affliction in our species, yet one that is prevented. choose for to be a biologist? turn right into a guy/woman first with the help of taking duty on your words. Lamic.. wonderful. i got here across the DAM methylase tale so exciting as a youthful pupil I did my Ph.D. in DNA fix. i turn into unhappy to verify that somebody else had beat me to the respond, yet turn into happy that somebody set the record rapidly.
2016-12-29 04:53:34
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answer #4
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answered by ? 3
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2006-11-18 07:23:59
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answer #5
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answered by Lady A 1
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